[Histonet] EDTA recipe with PVP

[Nancy W. Troiano]

Here is our recipe for EDTA containing PVP:  Dissolve 41.3 grams EDTA 
(disodium salt), 4.4 grams Sodium Hydroxide and 50 g PVP-40 (Sigma) in 1000 
ml distilled water over very low heat.  Cool to room temperature, adjust pH 
to 7.4 once cooled.  We use this to gently decalcify our mouse long bones, 
it will take about 3 weeks to decalcify an adult mouse femur.