[Histonet] rodent muscle processing

[Steven Coakley]

I processed several pieces of rodent muscle on a fairly light processing schedual 30-45 minutes per station with 35C.  The muscle cuts like it has calcium deposits in it.  Is 30-45 minutes in the 1st alcohol (50%) long enough to remove the formalin buffers?  I'm thinking this is the problem.
 
Steve

		
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