[Histonet] PMMA sections sticking to plastic

[Matt Prideaux]

Hi,

We had a similar problem on our LR White resin sections. Have you tried leaving
the slides to cool for a couple of hours after they have been removed from the
oven? this seemed to solve the problem for us. Also what type of plastic
wrapping do you use? we found that saran wrap seemed to work fine whereas using
a thicker plastic wrap caused problems when we tried to remove it.

Hope that helps

Matt


-- 
Matt Prideaux
Academic Unit of Bone Biology
Division of Clinical Sciences (South)
D Floor (DU20) Medical School
Henry Wellcome Laboratories for Medical Research
University of Sheffield
Beech Hill Road
Sheffield 
S10 2RX





Quoting "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>:

> We're having some problems with our PMMA sections adhering to the
> plastic instead of the glass slide.  After sectioning, we place the
> sections on a plus slide (+ charged) flooded with 50% alcohol.  We
> stretch the section, place a strip of plastic wrap over it, and roll it
> flat.  We will then stack 10 or so slides like this together with tongue
> depressors at the top and bottom, and clamp them together.  We will
> leave them in a 60 degree oven overnight (at least).  After we remove
> them from the oven, we are unable to peel the plastic off the slides
> without the sections coming off as well.
> 
> The last time this happened, I proceeded with deplasticizing using
> xylene and MEA (recommended by the Technovit instructions) and kept the
> plastic wrap on the slides.  I was able to remove it by eventually
> placing the slides in acetone.  Is there an easier way?
> 
> Best wishes,
> 
> Teri Johnson
> Managing Director Histology Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, MO 64133
> 
>