Fwd: [Histonet] blood sample for TEM...

[Ian Montgomery]

         For TEM I would simply put a couple of drops of blood into 
the primary fixative. Agitate gently, leave for 30 minutes then spin 
down. Decant off the supernatant and replace with several buffer 
washes before the post fixative. Dehydrate and embed in epoxy resin. 
If the pellet started to breakdown during processing then another 
spin solved the problem.
Ian.



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>Date: Tue, 23 Aug 2005 01:03:20 +0900
>From: Ul Soo Choi <cytologer <@t> gmail.com>
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>Hi histonetters!
>
>I am interested and in need of a protocol for preparing blood samples
>for transmission electron microscopy. But I am short of appropriate
>protocol for fixation, and processing for it.
>
>I would like to see cat neutrophils and lymphocytes by TEM, and
>
>Would anyone there who has experiences in blood specimen for TEM, kindly
>give me some tips for preparing blood samples for EM?
>
>Thank you for any inputs. I would appreciate.
>
>Best regards,
>
>Ul Soo Choi
>DVM., PhD.
>Dept of clinical pathology,
>CVM, SNU, Seoul, Korea
>82-02-880-8688
>
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Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07623 975451
e-mail: ian.montgomery <@t> bio.gla.ac.uk