[Histonet] Re: Brain sections-rat

[Favara, Cynthia (NIH/NIAID)]

I would be happy to help with suggestions but need a bit more precise
information.

The information provided indicates you are cutting fixed embedded tissue. Is
this formalin fixed paraffin embedded, standard protocol? Your problems may
be inadequate fixation/processing. I have been doing rodent tissue for years
and with brain I have found that inadequate fixation leads to a myriad of
problems.

There have been numerous discussions of rodent brain
fixation/processing/cutting over the years that you should be able to find
in the archives. 

If you still have questions please fell free to contact me.

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: kotresh mattur [mailto:kotreshmattur <@t> yahoo.com] 
Sent: Saturday, August 20, 2005 5:45 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Brain sections-rat

 Dear all,
            We are facing some problems in rat brain sectioning and
staining. We are sectioning brain at 5-6 microns thickness and then we take
sections at 37-40 centigrade after leaving for 1min. in water bath or till
the sections are flattened. During staining at hydration step sections are
lifting off. For this problem we tried to take the sections on histogrip
coated slides which also did not help us at all. Whether there are any
special delicate techniques in brain sectioning, staining? Regarding this
problem can anybody help with their vast experience? Suggestions/advice from
all are welcome.

 Thanks in advance

Dr.K.Y.Mathur



Dr.K.Y.Mathur
Junior Scientist
Dept.of Preclincal Safety Evaluation
Discovery Research
Dr.Reddy's Laboratory Ltd.
Bollaram Road, Miyapur,
HYDERABAD-INDIA-49.
 
Ph No: 
040-23045439-exn 424,421,422,423
Cell :09391293129

		
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