[Histonet] Fixative for storage of perfused rat organs

[Charles Scouten]

  Tip:  to preserve the extracellular space for EM of soft tissue, use the Perfusion One, and associated protocol.  It was originally developed for EM.
http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=471001&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=Sacrifice+Equipment&idsubcategory=21

Don't know about the storage question.


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clover Daley
Sent: Thursday, August 18, 2005 4:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fixative for storage of perfused rat organs

I am putting a tissue bank together for research students.
We are currently perfusing with 2% paraformaldehyde and 1% glutaraldehyde in phosphate buffer to collect tissues for EM studies.
The liver, kidney, stomach, brain will be stored. What fixative and temperature of solution would be best.
These tissues will be used for light microscopy studies.
Also we will be perfusing with 4% paraformaldehyde in phosphate buffer, harvesting the same tissues. Should they be stored in the same solution and do they need to be kept in the refrigerator?

Thank you for your time,
Clover Daley
Palmer Center for Chiropractic Research
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