[Histonet] Ahh, my trichromes are green again

[Hofecker, Jennifer L]

Hi everyone,
Just wanted to thank the many people who gave me helpful hints.  We found that our commercially prepared Gomori trichrome solution was at pH 2.0!  We brought it up to 3.4 and it worked great.  We have ordered new solution and I plan on checking the pH when it comes in.  We also decided to wait a few minutes after cutting frozen sections.  Several people recommended both of these things and we are very grateful.
Have a great rest of the week.
 
Jennifer
 
 
 
Jennifer Hofecker, HT (ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
(615) 343-0083
(615) 343-7089 fax