[Histonet] Breast Processing Protocol

[Rogerson Kemlo (ELHT) Pathology]

Sometimes, with fatty tissue, it is useful to go through a fat solvent
and then to re-fix. What I used to do was to dehydrate, then go through
xylene (which is a fat solvent, then back to 100% alcohol (which is also
a fixative) then through xylene to wax. The results were better but I
never understood if it was due to the extra processing or the removal of
fat.

Anyway the logic of it appealed to me. There are other fat solvents you
could try.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
DDDeltour <@t> mar.med.navy.mil
Sent: 17 August 2005 14:58
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Breast Processing Protocol

I am looking for a program for processing fatty breast tissue. We
currently
let the breast sit in cassettes in Pen-Fix for an extra day before
processing. We then process the first Fixative in Pen-Fix for one hour
then
in 10% Formalin for the second hour (I do not like this but that is they
way
it was done when I got here). The next steps are.. 95% one hour X3, 100%
one
hour X3, Xylene 1 hour X2. Paraffin for one hour X3. I am considering
replacing one of the 95% with another fixative. What programs do you use
for
this? They don't like to hear Sh in Sh out:-). Thank you!

 

DOUGLAS D. DELTOUR 
HISTOLOGY TECHNICIAN 
NMC PORTSMOUTH VA 

 

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