[Histonet] antigen retrieval for c-fos or preproenkephalin

[Carolyn Johnson]

Hello,

I am doing indirect immunofluorescence on 4% paraformaldehyde fixed (5
hours) mouse tissue, cut to 40 um.  I cannot achieve labeling for either
c-fos (Chemicon) or pre-proenkephalin (bachem).  Has anyone had experience
with either of these antibodies?  Also, the companies recommended that I try
antigen retrieval.  If anyone has a protocol that works for either of these
antigens, I would greatly appreciate it!

 

Thanks,

Carolyn