[Histonet] aldehyde fuchsin

[Marshall Terry Dr, Consultant Histopathologist]

John tells us:
"It is also possible to recover
the aldehyde fuchsine as a precipitate that can 
be collected by filtering, dried and stored
for 10 or more years."

OK John ......how?
(This would solve my problem of needing it every 4 months only and suffering the groans of the techs at having to make it up again)

BTW - I am sure that someone suggested a paraldehyde substitute at one time, but I omitted to keep the post.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: John A. Kiernan [mailto:jkiernan <@t> uwo.ca]
Sent: 17 August 2005 16:11
To: Histonet
Subject: Re: [Histonet] aldehyde fuchsin


The solution of aldehyde fuchsine has a rather
short shelf-life (8 weeks) so it's best to make 
up your own. It is also possible to recover
the aldehyde fuchsine as a precipitate that can 
be collected by filtering, dried and stored
for 10 or more years. Solutions made from aldehyde
fuchsine powder (0.125% in 70% ethanol with 
1% acetic acid) are more stable than the traditional 
directly made solution, being good for about 2 years. 

It is said that solutions of aldehyde fuchsine
made from the powder will not stain pancreatic
islet B cells without prior permanganate oxidation 
(see Mowry,RW 1978 Stain Technol. 53: 141-154. This
paper also has useful tips for other uses of
aldehyde fuchsine. 
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Robin Newlin wrote:
> 
> Hi All
> Does anyone know if aldehyde fuchsin is available commercially, or do I have to brew my own.
> Thanks
> Robbin Newlin
> Manager, Cell Imaging/Histology Core Facility
> The Burnham Institute
> 10901 N. Torrey Pines Road
> LaJolla, Ca. 92037
> 858-646-3100 ext.3552
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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