[Histonet] IF in bone or cartilage paraffin sections

[pex]

Hello, all.
 
I have done immunofluorescence in bone or cartilage paraffin sections 
for several months. I use normal immunofluorescence protocol for 
staining, but I have tried different methods for antigen retrieval, finally I 
think that 0.1% trypsin (15min or 20min) for antigen retrieval in my 
experiments is a little bit good.
In addition, I have already used different antibodies for 
immunosatining. One of them is working well, positive expressions can be found in 
cartilage, osteoblasts and osteocytes, but others express in cartilage 
strongly, however they are very weak in bone( but they express stronger 
in primary osteoblasts).I do not know the reasons!
 
Any suggestions will be useful for me! 
 
Thank you!
 
Guofeng



Guofeng
		
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