[Histonet] more about the gomori's on Frozen muscle

[Hofecker, Jennifer L]

First, 

Thank you to those of you that responded. Especially those who understood that when doing modified Gomori's for a neuropathologist, muscle should stain green. I realize that in FFPE, muscle stains red, however our muscle has been staining correctly (green) until this week. I think that over the past several years, the neuropathologist might have noticed if we were staining the muscle the completely wrong color. (sorry, a little sarcastic) 

I am also not fixing the sections at all. We go right into hematoxylin. A private reply I received suggested that putting fresh slides in the freezer may be acting as "fixation" (lyophilization) thus, the fresh cuts aren't getting fixed. To those of you doing neuro muscle staining (remember, we're searching for normal to be GREEN) do you use fixation before staining?

I would like to remind some of you that histonet is a tool for us to help each other out. It really is not for use as a weapon. I honestly needed advice, but I as usual, hesitated because I was afraid of the "less than kind" responses that seem to come with many of my posts. 

Thanks again to those who were kind enough to help me out. There is such a wealth of knowledge out there in histoland. Hopefully somebody will have the answer. 

Bracing for the next round, 

Jennifer

 

Jennifer Hofecker, HT (ASCP) 

Vanderbilt University Medical Center 

Division of Neuropathology 

(615) 343-0083 

(615) 343-7089 fax