[Histonet] Problems with Gomori's Trichrome on Frozen Muscle

[Kristen Broomall]

Paula,

It is supposed to be greenish.

Modified Gomori's Trichrome method for muscle biopsies:

Nuclei - red/purple
Normal Muscle myofibrils - green with distinct A & I bands
Intermyofibrillar muscle - red
Interstitial collagen - green

Jennifer,

We just got a weird red artifact (big splotch of red in the middle of the
section) the other week on a biopsy, but it had been cut on another cryostat
other than ours. We thought it may have been due to a dull blade or
something like that. We recut it on our cryo & it stained fine. 

I hope you get it worked out & I'm interested to know what's going on! Good
luck.

Kristen Broomall, HT (ASCP)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Paula
Pierce
Sent: Friday, August 12, 2005 3:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Problems with Gomori's Trichrome on Frozen Muscle


Results for Gomori's Trichrome Stain:
 
Cell cytoplasm, MUSCLE - Red
Collagen - Green
Nuclei - Blue to Black
 
 


Paula Pierce, HTL(ASCP)HT

Excalibur Pathology, Inc.
630 N. Broadway
Moore, OK 73160
405-759-3953
contact <@t> excaliburpathology.com
www.excaliburpathology.com
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