[Histonet] beta-galactosidase in mouse embryos

[Anna Elisse Beaudin]

Hi all,

   I am planning to do beta-galactosidase staining on dpc 9.5 mouse
embryos, and I was wondering if anyone had any advice on the best
fixative to use prior to staining, as well as the best clearing agent
to use after.  I've seen many protocols that call for one hour 4%
paraformaldehyde fix, but when I have done Bgal stain on adult tissue
and tissue sections, this fix seems to reduce staining.  I was also
planning to use 100% methyl salicylate as a clearing agent.  I'd very
much appreciate anyone's advice!

thanks in advance,
Anna Beaudin
Division of Nutritional Sciences
Cornell University