[Histonet] Cryostat sectioning of mammary gland

[Bonner, Janet]

 The Leica cryostats we use(CM1900) have a "snowflake" button to bring the
temperature of the chuck holder to -50 degrees real quick. It is very useful
when we get specimens like these!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
To: Tessa Murray; histonet <@t> lists.utsouthwestern.edu
Sent: 8/11/2005 10:21 AM
Subject: RE: [Histonet] Cryostat sectioning of mammary gland

Dear Tess,

Are you sure the cryochamber is below -30ºC or just the specimen is at
that temperature?? As your problem indicates that the knife & anti-roll
plate are too warm, if the specimen is at -30ºC. With the correct
temperatures, mammary gland should be easy to section.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Tessa Murray [mailto:tessajmurray <@t> hotmail.com] 
Sent: 10 August 2005 21:50
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cryostat sectioning of mammary gland



   I'm looking for some advice on cryosections.  We are trying to
collect
   frozen  sections of mammary gland tissue and are experiencing
problems
   - we do a lot of paraffin work here so the cryostat technique is
quite
   new to us.  We find that the knife cuts the OCT fine, but when it
hits
   tissue  it does not cut so we get sections with tissue shaped holes
in
   them.   We've  tried  changing the block and knife temp (we have
tried
   altering  them  between  -30  to -35C) but nothing seems to help.
Any
   advice appreciated...

   tess
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