[Histonet] Orcein for copper-binding protein/Hep B

[Lee & Peggy Wenk]

Are you looking for copper or for the hepatitis B?

If I remember correctly, orcein stains the hepatitis B inclusion body (the
ground glass appearance on an H&E)and it also stains elastin. Other stains
for the hepatitis B were the aldehyde fuchsin and the Victoria blue. I think
most labs do an IHC for hepatitis, as you can differentiate Hepatitis B core
from Hepatitis B surface antigens, as well as differentiate it from
Hepatitis A, C or D.

None of these stained for copper, such as in Wilson's disease. For that, we
use rhodanine. I believe it did not stain the actual copper, but rather the
protein to which copper was bound.

Let me know what procedures you need.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak MI 48073

 


Lee & Peggy Wenk
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Malam
Jacqueline
Sent: Wednesday, August 10, 2005 6:55 AM
To: Histonet submissions (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] Orcein for copper-binding protein/Hep B

Hello Histonetters.
Has anyone got a really good orcein method for copper-binding protein/Hep B,
and is there a recommended brand or vendor of the dye? Would you use
synthetic or natural (if you can still get it)?
Cheers
Jacqui Malam
Lancaster Infirmary
N England



DISCLAIMER: This e-mail is confidential and privileged. If you are not the
intended recipient please accept our apologies; please do not disclose, copy
or distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful. Please
inform postmaster <@t> rli.mbht.nhs.uk that this message has gone astray before
deleting it.  Comments or opinions expressed in this email are those of
their respective contributors only. The views expressed do not represent the
views of the Trust, its management or employees. Morecambe Bay Hospitals NHS
Trust is not responsible and disclaims any and all liability for the content
of comments written within.Thank you for your co-operation.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet