[Histonet] cytospin preps and plus slides

[Emily Smith]

Hello  HistoNetters!

What percentage cell recovery is expected/observed/acceptable for cytospin
or for sedimentation techniques?

Thank you!
~Emily

Emily Smith
Ikonisys Inc.
5 Science Park
New Haven, CT 06511

----------------------------------------------------
Message: 17
Date: Thu, 4 Aug 2005 15:29:14 +0100
From: "Rogerson Kemlo \(ELHT\) Pathology" <Kemlo.Rogerson <@t> elht.nhs.uk>
Subject: RE: [Histonet] cytospin preps and plus slides
To: "Elizabeth Chlipala" <liz <@t> premierlab.com>,	"Histonet"
	<histonet <@t> pathology.swmed.edu>
Message-ID:
	<53A22BBB01D6184EBF7A4DC18A021E9E698E5C <@t> elht-exch1.xelht.nhs.uk>
Content-Type: text/plain;	charset="us-ascii"

Maybe you need to spin faster and/or add protein to the vial to act as
an adhesive; you may have better success with Cytyc LBC.

Try the adhesive, bovine albumin, that may 'glue' ore of the cells onto
the slide but I think you will always get cell loss with the filter
paper. I found sedimentation techniques to be more successful.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Elizabeth Chlipala
Sent: 03 August 2005 19:43
To: 'Histonet'
Subject: [Histonet] cytospin preps and plus slides

Hello Everyone

I'm working on a project for a client who is trying to isolate prostate
epithelial cells.  I'm getting the cells submitted to me fixed in 10%
NBF in eppendorf tubes.  I'm using a cytospin for 5 minutes at 1000 rpm
and plus slides.  The problem is that I'm not getting the correct yield
of cells.  They check the number of cells in the prep prior to me
receiving them.  They are submitting numbers of cells in the thousands,
but when I prepare the cytospin slides, I'm only getting cells numbering
in the hundreds and sometimes less.  Does anyone have any suggestions?

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com

Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309