[Histonet] CD4 CD8 stain
cmeiers <@t> thelabrat.com
Sat Apr 23 08:56:53 CDT 2005
Could someone please give advice regarding cd4 and cd8 staining of
brain tissue. We dissected out the brain of a mouse, made
longitudinal section midbrain, one half of the brain was placed in
formalin for routine H & E, the other half was snap frozen for the cd4
and cd8. The sections came out absolutely horrible with what looked
like exploded tissue. Should the brain be treated in a different
fashion ? Would formalin interfere with staining CD4 and CD8 ?
I know this probably sounds like a very elementary question, we are
currently waiting for a new Histo tech that is well versed in Immuno
staining, but in the meantime research carries on.
I am thanking you in advance and would appreciate any comments. As
you have guessed, I am not a Histologist, just someone trying to keep
the lab going until we fulfill the vacancy.
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