[Histonet] Stain for Proteoglycans
juan.gutierrez <@t> christushealth.org
Thu Apr 21 06:05:02 CDT 2005
Back in my biochemistry research days, we use to stain proteoglycans with coomassie blue for the protein core and alcian blue for the sulfated carbohydrate chains. This was done on gels, but you might try it on tissue. Good luck.
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
My opinions are my own and do not reflect those of my employer. Long live free speech!
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John A. Kiernan
Sent: Wednesday, April 20, 2005 1:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Stain for Proteoglycans
If you simply want to stain all proteoglycans, use alcian
blue at pH 2.5. It will bind to the glycosaminoglycan
components, which make up the bulk of proteoglycan
molecules. Alcian blue will also stain heparin (mast
cell granules), but they are unlikely cause confusion.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
NIDAL E MUVARAK wrote:
> Is there a stain we could do to assess proteoglycan content in the mouse
> pulmonary arteries?
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