[Histonet] Stain for Proteoglycans

[GUTIERREZ, JUAN]

Back in my biochemistry research days, we use to stain proteoglycans with coomassie blue for the protein core and alcian blue for the sulfated carbohydrate chains.  This was done on gels, but you might try it on tissue.  Good luck.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long live free speech!


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John A. Kiernan
Sent: Wednesday, April 20, 2005 1:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Stain for Proteoglycans

If you simply want to stain all proteoglycans, use alcian
blue at pH 2.5. It will bind to the glycosaminoglycan
components, which make up the bulk of proteoglycan
molecules. Alcian blue will also stain heparin (mast 
cell granules), but they are unlikely cause confusion. 
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
NIDAL E MUVARAK wrote:
> 
> Is there a stain we could do to assess proteoglycan content in the mouse
> pulmonary arteries?
> 
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