[Histonet] rabbit anti-GFP (Molecular Probes)

[Anni-Maija Linden]

Hi All, 

Do you have a simple method for GFP detection on mouse 4%PFA perfusion 
fixed free-floating brain sections using rabbit serum anti-GFP (Molecular 
Probes, A6455) and ABC-DAB detection. I would like to know how to block 
peroxidases effectively and which blocking buffer works best to get low 
background. Also optimal concentration of primary antibody? Thanks a lot.

Best Regards, Anni-Maija 
-- 
Anni-Maija Linden, Ph.D.
Institute of Biomedicine, Pharmacology
University of Helsinki
POB 63, 00014 University of Helsinki
Finland

tel. 358-9-191-25357 or 358-50-3441574 (gsm)
email: anni-maija.linden <@t> helsinki.fi