[Histonet] Stain for Proteoglycans

[John A. Kiernan]

If you simply want to stain all proteoglycans, use alcian
blue at pH 2.5. It will bind to the glycosaminoglycan
components, which make up the bulk of proteoglycan
molecules. Alcian blue will also stain heparin (mast 
cell granules), but they are unlikely cause confusion. 
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
NIDAL E MUVARAK wrote:
> 
> Is there a stain we could do to assess proteoglycan content in the mouse
> pulmonary arteries?
> 
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