[Histonet] Mallory PTAH and Zenkers

lpwenk <@t> sbcglobal.net lpwenk <@t> sbcglobal.net
Sun Apr 17 04:28:25 CDT 2005

No, you would not have to do the iodine and sodium thiosulfate step if you
fix/post-mordant in something other than mercury.

If you want, fix the tissue in formalin, process/embed/section as usual, and
then postmordant in Bouin at 60 degree C for 1 hour before staining. Same as
doing a trichrome. Wash in running water afterwards until the yellow color
is gone. Stain with PTAH. Works great.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 12, 2005 6:04 PM
Subject: [Histonet] Mallory PTAH and Zenkers

Hello, I have a question about the Mallory's PTAH.  If I use the zenkers
without mercuric chloride do I still need to use Gram's iodine and
sodium thiosulfate?  I assume not but need to be sure.

Thanks for the help.

Margaret Perry HT

South Dakota University

Veterinary Science


Brookings SD

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