[Histonet] Tape transfer sections and IHC

[Cory Collins]

Sorry for not being more specific, you don't have to read the entire paper,
scroll down to page 34 and take a look under the header of "Tissue Transfer
Technique".

Cory

-----Original Message-----
From: John Baker [mailto:bakerj <@t> umich.edu]
Sent: Friday, April 15, 2005 2:18 PM
To: Cory Collins
Subject: Re: [Histonet] Tape transfer sections and IHC


Connie, fyi- ?  I did not print out the 20 page paper but you may want  
to look at it.  Have a fun weekend!  John

On Apr 15, 2005, at 2:35 PM, Cory Collins wrote:

> Anita,
>
> We use a different type of tissue transfer using a substance called  
> quick
> mount.  It's a great help because we can  cut the tissue from one  
> slide and
> transfer it to numerous other slides for IHC and we get great results.  
>  My
> pathologist, Dr. Rodney T. Miller, has written a protocol for this  
> technique
> at http://www.ihcworld.com/_books/Technical-IHC.pdf .  If you have any
> questions you can e-mail me at ccollins <@t> propathlab.com.
>
> Cory
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Anita
> Jennings
> Sent: Friday, April 15, 2005 11:38 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Tape transfer sections and IHC
>
>
>
>
>
>
> I had problems with a 'matrix' looking  background and Bernice said  
> they
> were working on  a special mounting media. I know she checks the  
> histonet
> so hopefully she will answer here.
>
>
>
>
>
>
>
>
>
>
>
> I am doing hrp IHC on tape transfer sections and I am experiencing a
> problem
> with unwanted staining on the coating on the polymer coated slides.   
> Does
> anyone else out there do IHC on the tape sections experience this  
> problem?
> Please suggest a solution to this.
> Thank you,
> Patsy
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John A. Baker
Histology Unit
Orthopaedic Research Laboratories
The University of Michigan
400 North Ingalls, G-161
Ann Arbor, MI 48103-0486
office phone:734-936-1635

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