[Histonet] Temp/Humidity Records?

[Pamela A. Marcum]

The comment for the records were regarding IHC and yet I find changes in
humidity and temperature have more effect on my sectioning and mounting of
paraffin and plastic sections.  If you are doing automated IHC I don't see how
this would matter.  If it is manual and you use a humidity chamber it still
won't matter much.  I could only see this mattering to IHC if it is done on the
bench in open air.  The changes I get seasons change throughout the day as heat
or AC are added or changed.

Is this really a new requirement??


Pam Marcum




Quoting "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>:

> So you keep accurate records.
> What can you do with this information that is actually useful?
> 
> Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
>  Consultant Pathologist
>  Rotherham General Hospital
>  South Yorkshire
>  England
>         terry.marshall <@t> rothgen.nhs.uk
> 
> -----Original Message-----
> From: Breeden, Sara [mailto:sbreeden <@t> nmda.nmsu.edu]
> Sent: 15 April 2005 14:30
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Temp/Humidity Records?
> 
> 
> During a recent inspection, the SOP and documentation for room
> temperature/humidity in the histo lab were noted as being absent.
> Although I document instrument temperatures, I have not kept records on
> room temp/humidity. It was noted that these factors may affect IHC
> staining quality.  If I am to begin documenting these parameters, I'd
> like to know if there are any guidelines in a general sense (or a
> specific one) relating to the variances and requirements.  Any help
> would be appreciated!
> 
> Sara A. Breeden, HT(ASCP)
> New Mexico Department of Agriculture
> Veterinary Diagnostic Services
> POBox 4700
> Albuquerque, NM  87196
> (505)841-2576
> (505)841-2518 FAX
> 
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