[Histonet] Long and short biopsy runs with one processor
Bill Sinai
bills <@t> icpmr.wsahs.nsw.gov.au
Thu Apr 14 16:35:31 CDT 2005
We occasionally run cell blocks but most times they are put through the
routine overnight processing.
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774
-----Original Message-----
From: dahmed <@t> mdanderson.org [mailto:dahmed <@t> mdanderson.org]
Sent: Thursday, 14 April 2005 11:13 PM
To: Bill Sinai
Subject: RE: [Histonet] Long and short biopsy runs with one processor
What about cell blocks? Do you feel that your currenct processing schedule
for biopsies would be appropriate for cytology cell blocks? Our current
bx/cell block protocol is 20 minutes in all stations including formalin for
a total run time of 6 hours? Can I trim this back a little?
David S. Ahmed
Chief Histology Technician
Department of Pathology
M.D. Anderson Cancer Center
"Bill Sinai"
<bills <@t> icpmr.wsahs.nsw.gov.au> To:
Sent by: "'Roxanne Soto'"
<godsgirlnow <@t> MSN.COM>, "histonet (E-mail)"
histonet-bounces <@t> lists.utsouth
<histonet <@t> lists.utsouthwestern.edu>
western.edu cc:
04/13/2005 04:55 PM
Subject:
RE: [Histonet]
Long and short biopsy runs with one processor
Roxanne,
Most are the small GE biopsies >3mm in diamater or "thin" slices of any
urgents >3mm thick.
Solution Time
Formalin 0min
70%ethanol 5min
95% 5min
100% 5min
100% 10min
100% 10min
Xylol 5min
Xylol 5min
Xylol 10min
Wax 5min
Wax 10min
Wax 10min
Temperature ambient with Pressur/Vacuum on all stations.
Two Leica ASP300 and two VIP 3000
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774
-----Original Message-----
From: Roxanne Soto [mailto:godsgirlnow <@t> MSN.COM]
Sent: Thursday, 14 April 2005 7:37 AM
To: Bill Sinai
Subject: Re: [Histonet] Long and short biopsy runs with one processor
What size is the tissue on the 2 hour run? How long is each station?
What kind of processor? Do you use the vacuum?
Roxanne
----- Original Message -----
From: Bill Sinai
To: histonet (E-mail)
Sent: Wednesday, April 13, 2005 5:30 PM
Subject: RE: [Histonet] Long and short biopsy runs with one processor
Julia,
I agree with your philosophy about small runs. We are a pathology
department supporting a large teaching hospital and an even larger area
pathology service. However, we also do a considerable amount of
private
work for several endoscopy clinics in our locality.
The scenario you describe is very similar to ours.
We have the usual overnight run, usually 450-600 blocks with the large
material in one processor and the smaller in another. We receive the
endoscopy specimens anytime from 7:30am through to 3:00pm each day,
with
any
pick ups after 6:00pm being processed the next morning in a short run
2-2.5hrs. This means we can have at least two runs per day of
endoscopy
specimens. The endoscopy specimens from the private clinics are about
30%
of our work and the TAT for these specimens is >2 days from pick-up to
hard
copy result to the clinician.
We also process renal biopsies several times per day as well as the
occasional urgent specimen.
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Julia
Dahl
Sent: Thursday, 14 April 2005 4:13 AM
To: Joyce.Rush <@t> sjmcmn.org; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Long and short biopsy runs with one processor
Joyce -
First let me preface with my bias - I am a GI pathologist (read small
biopsy
material is what I live and breathe).
Long processor runs are great for standard surgical material - but
absolutely overprocess small pieces of tissue, resulting in hard,
dehydrated, difficult to cut little brittle fragments.
The best approach that I've seen and used is to examine your
"bottlenecks."
The main bottlenecks are the points at which you have lots to do in
front of
you - with limited resource to do it (i.e. you have 150 cassettes to
embed
coming off of the processor at one time and ONE embedding station.
That's a
bottleneck.)
What time do you usually start your processor? Say your processor
starts at
10:00 p.m. with a standard 6 hour process run. Your pathologists or
your
PAs close the grossing stations at 6:00 p.m. and load the processor -
and
everything sits there for 4 hours (that's another bottleneck).
In this scenario - you can add a second processor run at virtually any
time
that you are staffed that allows you to turn over the processor for the
standard run by 6:00 p.m. (when the standard next load is).
Since a small biopsy run usually takes about 2 hours on the Vitek:
0600 - Standard processor emptied - large surgical cases ONLY (about
2/3
of
your blocks)
0600 - 1000: Embed, cut, stain and coverslip surgicals
0700 - 0800: Previous day's and early AM courier run of small biopsies
grossed in
0800 - 1000: Small biopsy processor run
1000 - small biopsy processor emptied
1000 - 1230: Embed, cut, stain and coverslip biopsies.
If you wanted to do another biopsy run - to allow same day turnaround
time -
the 2nd biopsy run could be scheduled at 10:30 each day with slides
coming
out around 3:30.
I'm interested to hear other people's ideas.
Julia
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>Subject: [Histonet] Long and short biopsy runs with one processor
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>We are a community hospital and last year processed 28,000 blocks. We
are
>being challenged by our Laboratory Director to run a long and short,
biopsy
>run daily, M-F. Currently we run everything together. We have one
>processor, a VIP5. I'd love to hear of ways people have handled this.
>Thanks so much! I always get such good advice from this group!
>
>Joyce
>
>Joyce A. Rush, BS, MT(ASCP)
>Laboratory Manager
>St Joseph's Medical Center
>523 North Third Street
>Brainerd, MN 56401
>Office:218-828-7500 Fax: 218-828-7510
>
>
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