Janet.Bonner <@t> FLHOSP.ORG
Wed Apr 13 09:46:39 CDT 2005
AND who knows what it does to the reactivity of the IHC staining...
From: histonet-bounces <@t> lists.utsouthwestern.edu
Sent: 4/13/2005 10:35 AM
Subject: [Histonet] isopropanol
I used isopropanol quite a bit years ago. It dehydrates satisfactorily,
although it is not as efficient as absolute ethanol. It leaves the
a little more brittle than ethanol does, so the sectioning is slightly
different. I never found any difference in speed, certainly not to the
degree you appear to be looking for. Our reasons for using it had to do
with the problems getting a license for using ethanol in Britain at that
In essence, any time you speed up processing, particularly for fatty
tissues, you will reduce quality. That is usually the trade off.
thinner blocks and using a fast fixative (like Carnoy or Clark) are
the only things that might help, and fixatives like that have their own
issues regarding the quality of the morphology in the finished
Essentially, what you are looking for has been done decades ago, and the
times and procedures we use now is the result.
----- Original Message -----
From: <dahmed <@t> mdanderson.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 13, 2005 5:36 AM
Subject: [Histonet] (no subject)
> Does anyone use Isopropyl Alcohol instead of Ethyl Alcohol on their
> processor? Does anyone feel it does a better job at dehydrating the
> tissue? At the request of the pathologists, I am looking for any
> that will speed up the processing time for breast tissue. Any
> and/or suggestion is welcome.
> Thank you.
> David S. Ahmed
> Chief Histology Technician
> Department of Pathology
> M.D. Anderson Cancer Center
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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