[Histonet] Background with tape transfer slides, IHC

Gayle Callis gcallis <@t> montana.edu
Tue Apr 12 14:08:01 CDT 2005


I have not had a problem.  I do add Tween 20 to all buffers and diluents 
and we only use 1/2X slides.  We find these hold bone perfectly but we do 
double flash to ensure no lift off.   The Tween is 0.05% but you can try 
and increase concentration higher, 0.2% or so, maybe even higher than 
that.  You may be experiencing ionic interaction of chromogen to the 
polymer, commonly occurs with plus charge slides also, i.e. ring around the 
section only you have tinted polymer.

I am assuming you are using DAB and that is the background??

I am doing hrp IHC on tape transfer sections and I am experiencing a problem
with unwanted staining on the coating on the polymer coated slides.  Does
anyone else out there do IHC on the tape sections experience this problem?
Please suggest a solution to this.
Thank you,

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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