Prada-Puentes Carlos (RW6) PAHNT
Carlos.Prada-Puentes <@t> pat.nhs.uk
Tue Apr 12 08:59:39 CDT 2005
I have been using WT1 with good results from Santa Cruz biotechnology. My problem is that after 6 months the antibody has become unstable and altough we have concentrated it the nuclear staining is weaker and weaker. The datasheet does not recommend freezing and gives ONLY ONE YEAR!!!! Of guaranteed stability kept at 4 C..Should I freeze it when the new vial comes? Considering we use at a dilution of 1/3000 we need to freeze before we use it all.
Thank you to all
Dr Carlos Prada-Puentes
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