[Histonet] RE: Is TUNEL a waste of time?
sakima <@t> bigpond.net.au
Mon Apr 11 17:35:27 CDT 2005
On 11/04/2005, at 7:54 PM, Edwards, R.E. wrote:
> We abandoned the TUNEL method, many years ago as we felt it
> gave too many false +ves, we used H <@t> E staining until reliable
> antibodies came along, e.g., caspase 3............
I have found others who use TUNEL staining express a similar opinion
about the false positives. I would be interested to know if others too
have found an excessive rate of false positives.
Still the TUNEL method seems to be viewed by a great many as some sort
of Gold Standard. Many find still swear by it as a very good tool.
Regarding the issue of specificity - does caspase-3 also help to
distinguish between apoptosis and necrosis I wonder?
Centre for Transplantation and Renal Research
Westmead Millenium Institute
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