[Histonet] Is TUNEL as waste of time?
sakima <@t> bigpond.net.au
Sat Apr 9 07:26:59 CDT 2005
I am looking into the Roche TUNEL kit and have been getting various
opinions. Some say it is easy and reviewers will ask for a TUNEL stain
in preference to other methods such as staining for caspase-3. However
many people find that they get non-specific staining and even where
things seem to have gone well you get a great many cells that seem
absolutely healthy staining up positive.
Do people think that TUNEL is a sham technique that ivory tower
reviewers who never get their hands dirty in the lab ask for, never
realising how hopeless the technique is? Or is it still a genuinely
Centre for Transplantation and Renal Research
Westmead Millenium Institute
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