[Histonet] Mouse femurs and tibias fixation time

[Jackie M O'Connor]

I let them fix for IHC 24-48 hours.  Are you going to decalcify?  I 
decalcify in a formic acid decal, then trim away some of the cortical bone 
for the perspective I want (I'm doing whole legs). Since they bow to the 
right or left, I can get the surface I want.  Trimming away the cortical 
bone enables the processing solutions to penetrate much better, and I have 
no artifacts.

Jacqueline M. O'Connor HT(ASCP)
Assistant Scientist
GPRD Cancer Research
Abbott Laboratories, Abbott Park, IL
Jackie.OConnor <@t> abbott.com




"Grant, Debra" <DRG <@t> Stowers-Institute.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/08/2005 02:17 PM

 
        To:     "Histonet" <histonet <@t> lists.utsouthwestern.edu>
        cc: 
        Subject:        [Histonet] Mouse femurs and tibias fixation time


Hi all,

I am processing some mouse femurs and tibias for IHC. Any
recommendations as to amount of fixation time for Zinc formalin before
paraffin processing?

Thanks in advance!

Cheers! 

Debby Grant
Histology Specialist I 
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg <@t> stowers-institute.org
 

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