[Histonet] Smoothelin Antibody from Abcam
ctsblack <@t> capeheart.uct.ac.za
Fri Apr 8 03:03:17 CDT 2005
Perhaps somebody could help me with my problem??
I am trying to differentiate Myofibroblasts from Smooth Muscle cells.
SMC's will obviously stain positive with Actin,Desmin, myosin and
Vimentin. I want to do a Smoothelin stain, for which Myofibroblasts
are negative and thereby assume that I have identified Myo's.
I am using Abcam's Anti- Smoothelin, and using placenta and tonsil
controls trying to optimize this Ab. However....it is staining
TROPHOBLASTS and NOT Smooth Muscle Cells at all!!!!! I am getting
stunning Specific staining of the entire placental Trophoblasts, and
nothing on contrcatile vessels/arteries.
Has anybody used this antibody at all????? AB 8969 Anti- Smoothelin.
I would appreciate any adivice.
PS: I have referred this onto Abcam Technical Support and am waiting
for a reply.
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