[Histonet] Laminin antibody

[Dana Settembre]

Neeltje,
Try the antibody with no antigen retreival.
Dana Settembre
University Hospital -UMDNJ
Newark, NJ
USA

>>> "Coolen, Neeltje" <ncoolen <@t> burns.nl> 4/7/2005 4:36:35 AM >>>
Dear Histonetters,
 
Are there any people who have experience with the following
anti-laminin
antibody: mouse monoclonal, clone 4C7 from DAKO (code no: M0638)?
We tried to use this antibody on paraffin-embedded human skin tissue
(formalin-fixed), but it did not work. We tried several antigen
retrieval procedures, like pepsin, proteinase K and trypsin digestion,
but all of these fail.
Does anyone know an appropriate antigen retrieval or should I try
another antibody? 
 
Thank you for your help.
 
Neeltje Coolen, PhD student
VSBN
Zeestraat 26
1941 AJ Beverwijk
The Netherlands
 
 
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