[Histonet] Cryosectioning Whole Fish

[Greg Dobbin]

------- Forwarded message follows -------
From:           	Greg Dobbin <dobbin <@t> acad1.cs.upei.ca>
To:             	"Amber R Tompsett" <tompset2 <@t> msu.edu>
Subject:        	Re: [Histonet] Cryosectioning Whole Fish
Date sent:      	Wed, 6 Apr 2005 13:18:12 -0400

Hi Amber,
Would it be too disruptive to your study to inject a small amount of
cryomatrix intra-abdominally prior to freezing? I think the internal
organs, being small and fragile, are not sticking to one another 
when
frozen. 

I also suspect there is quite a high fat component in there as well,
so with fat, a colder cutting temperature is generally better.

By the way, I haven't tried the intra-abdominal injection technique
myself. Let me know if you try it and how well it works. Good luck.
Greg

From:           	"Amber R Tompsett" <tompset2 <@t> msu.edu>
To:             	histonet <@t> lists.utsouthwestern.edu
Date sent:      	Wed, 06 Apr 2005 11:07:34 -0400
Subject:        	[Histonet] Cryosectioning Whole Fish

> Hello Histonetters, 
> 
> I am currently trying to develop a method to cryosection whole
> Japanese medaka (a guppie-sized fish). We would eventually like to
> use labeled probes to do in-situ hybridization on the sections. We
> have tried sectioning with a disposable steel blade at multiple
> temperatures and section thicknesses. The muscle seems to section
> fine, but the organs in the body cavity and the brain (which are the
> parts of the fish we are interested in) shred or just completely
> disappear. 
> 
> Any ideas about what is happening or suggestions on how to get
> better sections? 
> 
> Thanks,
> Amber Tompsett
> Graduate Assistant
> Department of Zoology
> Michigan State University
> 
> 
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> Histonet <@t> lists.utsouthwestern.edu
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------- End of forwarded message -------


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin 
Research Technologist,
Pathology Lab,
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Happiness is a journey, not a destination.