[Histonet] Cryosectioning Whole Fish

[Amber R Tompsett]

Hello Histonetters, 

I am currently trying to develop a method to cryosection whole Japanese 
medaka (a guppie-sized fish). We would eventually like to use labeled probes 
to do in-situ hybridization on the sections. We have tried sectioning with a 
disposable steel blade at multiple temperatures and section thicknesses. The 
muscle seems to section fine, but the organs in the body cavity and the 
brain (which are the parts of the fish we are interested in) shred or just 
completely disappear. 

Any ideas about what is happening or suggestions on how to get better 
sections? 

Thanks,
Amber Tompsett
Graduate Assistant
Department of Zoology
Michigan State University