[Histonet] zincTris fixation on frozen sections

[Yves Heremans]

Dear All,

I am in the process of staining mouse kidney samples containing grafted 
mouse pancreatic islets with antibodies against MHC H2Dd and H2Db. 
Tissue samples available are either FFPE or unfixed, snap frozen. I 
decided to concentrate on the frozen samples due to the general 
difficulty of staining for surface markers after formalin fixation. My 
questions are:

1. If I want to use frozen sections, immediately fixed in zincTris 
(formalin-free), will I need to process the slides through increasing 
alcohols just as suggested for zincTris fixation of tissue samples ?
2. Will zincTris fixation yield better morphology compared to 
conventional acetone fixation ?

Yves Heremans
University of Minnesota
Stem Cell Institute
Tel 612-625-0964
Fax 612-624-2436

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