[Histonet] Re: Frozen sections fixed or unfixed for IFA
Gayle Callis
gcallis <@t> montana.edu
Mon Apr 4 11:12:33 CDT 2005
WE do it both ways with success but HOW you store and handle your sections
is important. I think we get our best fluorescent staining on fresh FS air
dried overnight, then fixed the next day followed by immediate IFA
staining. The sections MUST be dry before fixation, no matter which way
you handle them.
At 04:06 AM 4/4/2005, you wrote:
>Hi
>Asking this question for a colleague who does the flourescent staining
>on frozen sections.Does it make a difference to fluorescent staining if
>one fixes cryosections in acetone before freezing the slides at -80°C?
>Is it better to rather store the sections unfixed before freezing? Thank
>you
>Marilyn
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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