[Histonet] Re: Frozen sections fixed or unfixed for IFA

Gayle Callis gcallis <@t> montana.edu
Mon Apr 4 11:12:33 CDT 2005

WE do it both ways with success but HOW you store and handle your sections 
is important.  I think we get our best fluorescent staining on fresh FS air 
dried overnight, then fixed the next day followed by immediate IFA 
staining.   The sections MUST be dry before fixation, no matter which way 
you handle them.

At 04:06 AM 4/4/2005, you wrote:
>Asking this question for a colleague who does the flourescent staining
>on frozen sections.Does it make a difference to fluorescent staining if
>one fixes cryosections in acetone before freezing the slides at -80°C?
>Is it better to rather store the sections unfixed before freezing? Thank
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

More information about the Histonet mailing list