[Histonet] RE: Frozen sections

C.M. van der Loos c.m.vanderloos <@t> amc.uva.nl
Mon Apr 4 09:46:20 CDT 2005

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   size=2>Hi,</FONT></P>     <P><FONT    face=Times
   size=2>Good   question!   </P>   <P><FONT   face=Times
   size=2>Actually  we  never  tried  to  fix&n   acetone  before  storage  at  -80C. </FONT>The only t   know  is  that  proper  storage at -80C of unfixed cryostat section   works  well for a long time. Storing them unfixed gives you at least
   t   example,   antigens  preferabl   much  better  in  this  res   face=Times  size=2>Chris van der Loos, PhD<BR>D   Pathology<BR>Academical   Medical   Center   M2-230<BR>Meiber   gdreef         9<BR>NL-1105         AZ        Amsterdam<BR>The
   Netherlands</FONT><   size=2>phone:&nbsp;         +31        20        5665631<   BR>fax:&nbsp;&nbsp;&nbsp;            +31            20
   6960389<BR>e-mail:   href="mailto:c.m.vanderloos <@t> amc.uva.nl"   face=Times        size=2>c.m.vanderloos <@t> amc.uva.nl<   /FONT></A></P>          <P><FONT          face=Times
   size=2></FONT>&nbsp;</P>  <P><FONT  face=Times
   size=2>-----         Original         Message         -----</FO   NT></P></TD></TR>             <TR>             <TD
   align=right><B><FONT face=Times size=2>From&nbsp;   </FONT></B></TD>        <TD><FONT       face=Times
   size=2>Tyler                          &lt;mtyler <@t> uctgsh1.   uct.ac.za&gt;</FONT></TD></TR>    <TR>   <TD
   align=right><B><FONT face=Times size=2>Date&nbsp;   </FONT></B></TD>        <TD><FONT       face=Times
   size=2>Mon,      04      Apr      2005      12:06:16      +   0200</FONT></TD></TR>            <TR>            <TD
   align=right><B><FONT  face=Times  size=2>To&nbsp;   </FONT></B></TD>        <TD><FONT       face=Times
   size=2>histonet                          &lt;histonet <@t> lists   .utsouthwestern.edu&gt;</FONT></TD></TR>  <TR>
   <TD            align=right><B><FONT           face=Times
   size=2>Subject&nb   <TD><FONT    face=Times    size=2>[Histonet]    Frozen
   sections<   <P><FONT  face=Times size=2>Hi<BR>Asking this question
   for   sections.   if<BR>one  fixes  cry   slides  at  -80°C?<BR>Is  i   sections        unfixed       before       freezing?       Thank<   BR>you<BR>Marilyn<BR></FONT></P></BODY>

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