[Histonet] Secondary antibody-HRP conjugates

McNaughton, Euan euan.mcnaughtone <@t> agresearch.co.nz
Sun Apr 3 23:03:21 CDT 2005

Hi All,

Has anyone had any experience with Zymed SuperPicTure or other kits in
which HRP is directly conjugated to the secondary antibody? I can see
why it'd be useful for tissues that have high levels of endogenous
biotin, but I'm curious about their claims of "superior sensitivity" for
general use.  I'm quite new to the IHC game, but I thought that the
avidin-biotin system was used to improve senstivity, and so removing it
would have the opposite effect...

Any comments would be appreciated!


Euan McNaughton

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