[Histonet] PTAH Mordant

[Joe Nocito]

Ann Preece,
now that's going back to the good ole days.

Thank you all for your answers. The majority are using Bouin's as a mordant
and staining the usual way. That's why I love you all. Even an old goat
(cabrito here in Texas) like me keeps learning from y'all.

Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
lpwenk <@t> sbcglobal.net
Sent: Thursday, March 31, 2005 6:27 PM
To: Joe Nocito; Histonet
Subject: Re: [Histonet] PTAH Mordant


We use Bouin fixative - 60 degree oven for 1 hour (after deparaffinizing
slides and running them down to water). (Can skip the Lugolizing steps)

Then wash in running water until yellow disappears from the tissue (1-10
minutes, depending on type of tissue. The more striated muscle there is, the
longer it takes.)

Then stain as usual in PTAH.

We get better, more consistent staining with this method. One of my fellow
students found it when we were in training. We had to do a PTAH for our
practical. Worked great. I believe it was from the Ann Preece book.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Sent: Thursday, March 31, 2005 2:27 PM
Subject: [Histonet] PTAH Mordant


> Hey y'all,
> since we no longer have Zenkers because of the mercury, what is being used
> as a mordant for the PTAH stain?  My pathologists have been hounding me
all
> week, but with my other net postings and replies, I just forgot.  Thanks
> Histoland
>
>
> Joe Nocito, BS, HT(ASCP) QIHC
> Histology Manager
> Pathology Reference Lab
> San Antonio, TX
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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