[Histonet] Oxidising Agents in Haematoxylin[Scanned]

[John Kiernan]

Yes you could use the potassium salt instead.
If you oxidize with iodate, the staining solution
is not Ehrlich's haematoxylin. Paul Ehrlich (1886)
allowed his solution to oxidize in the air. That's
why it keeps for years & years.

John Kiernan
London, Canada
_____________________________________
Muskett David wrote:
> 
> Dear All
> 
> I am inquiring about the use of differing oxidising agents in histology.
> I am routinely using Sodium Iodate as the oxidising agent in Ehrlich's
> haematoxylin. Can the sodium iodate be substituted by Potassium Iodate?
> Is there a difference in their oxidising properties?
> 
> David
> 
> 
> David Muskett
> 
> Chief Biomedical Scientist, Histology
> 
> Royal Liverpool Children's NHS Trust
> 
> Eaton Road
> 
> Liverpool
> 
> L12 2AP
> 
> 
> 
> Telephone (0151) 293 3656
> 
> Fax (0151) 293 3617
>