[Histonet] Factor XIIIa
Julie.Sanders <@t> med.va.gov
Julie.Sanders <@t> med.va.gov
Thu Sep 30 12:40:07 CDT 2004
We get our from Cell Marque and have good results on the Ventana Benchmark.
Julie
Julie Sanders,BA,HT(ASCP)
Supervisor, Anatomic Pathology
VAMC Cincinnati, Ohio
-----Original Message-----
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Sent: Thursday, September 30, 2004 1:14 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 10, Issue 40
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Today's Topics:
1. RE: Red chromogens (C.M. van der Loos)
2. Re: Oxidising Agents in Haematoxylin[Scanned] (Gudrun Lang)
3. Mast Cell Stain other than Toluidine Blue (Featherstone, Annette)
4. multi-cassette search (Paula Pierce)
5. RE:Oil & Grease for the Leitz 1512 (mprice26 <@t> juno.com)
6. Re: RE:Oil & Grease for the Leitz 1512
(Don.Birgerson <@t> leica-microsystems.com)
7. Re: Red chromogens (Gayle Callis)
8. RE: Sucrose cryoprection (Charles Scouten)
9. Re: Sucrose cryoprection (Anila Syed)
10. Re: Mast Cell Stain other than Toluidine Blue (Gayle Callis)
11. FXIII antibody (Martha Ward)
12. RE: FXIII antibody (Jeff Gordon)
13. RE: FXIII antibody (May Wei)
14. FXIII antibody (Jason and Heather)
15. RE: FXIII antibody (Poteete, Jacquie A.)
----------------------------------------------------------------------
Message: 1
Date: Thu, 30 Sep 2004 13:27:42 +0200
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] RE: Red chromogens
To: histonet <@t> lists.utsouthwestern.edu
Cc: RFail <@t> Charleston.net
Message-ID: <1a7c2a1a8872.1a88721a7c2a <@t> amc.uva.nl>
Content-Type: text/plain; charset=us-ascii
Dear Rena,
Too much levamisole cannot result in no staining. If you have used one drop
(from the Dako concentrate??) per ml of substrate that should be OK (0.5 mM
levamisole is considered as optimal). Too much of levamisole may result in
somewhat weaker staining ultimately as levamisole inhibits the activity of
all AP isoenzymes except the one from intestinal tissue. Your AP label is
isolated from calf intestinal tissue.
I can strongly recommend you to test the new Dako Permanent Red substrate
(if already on the market).
One suggestion: if you are a PBS user the phosphate ions from that buffer
inhibit AP activity dramatically. Change to TBS or insert 2-3 washing steps
with a non-phosphate containing buffer between your final PBS washing step
and AP visualization.
Hope this helps.
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands
----- Original Message -----
>From Rena <RFail <@t> Charleston.net>
Date Wed, 29 Sep 2004 21:30:04 -0400
To histonet <@t> lists.utsouthwestern.edu
Subject [Histonet] Red chromogens
I have been having some trouble with some Abs stained with New Fuchsin
and permanent red. Recently I was asked if I were using Levamisole in
the permanent red and if so how much? I was told too much Levamisole in
permanent red would result in no staining. I have used 1 drop per ml for
both New Fuchsin and permanent red, which is the appropriate amount. We
have run both DABS and either permanent red or New Fuchsin with the
same AB from the same vial with vastly different results. Any ideas?
Rena Fail
------------------------------
Message: 2
Date: Thu, 30 Sep 2004 13:36:09 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: Re: [Histonet] Oxidising Agents in Haematoxylin[Scanned]
To: "Histonetliste" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000e01c4a6e1$ae7cbf80$eeeea8c0 <@t> server>
Content-Type: text/plain; charset="iso-8859-1"
We use Potassium Iodate in Mayers Hematoxylin.
Gudrun
----- Original Message -----
From: "Muskett David" <David.Muskett <@t> RLC.NHS.UK>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, September 30, 2004 12:35 PM
Subject: [Histonet] Oxidising Agents in Haematoxylin[Scanned]
Dear All
I am inquiring about the use of differing oxidising agents in histology.
I am routinely using Sodium Iodate as the oxidising agent in Ehrlich's
haematoxylin. Can the sodium iodate be substituted by Potassium Iodate?
Is there a difference in their oxidising properties?
David
David Muskett
Chief Biomedical Scientist, Histology
Royal Liverpool Children's NHS Trust
Eaton Road
Liverpool
L12 2AP
Telephone (0151) 293 3656
Fax (0151) 293 3617
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------------------------------
Message: 3
Date: Thu, 30 Sep 2004 07:37:30 -0400
From: "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org>
Subject: [Histonet] Mast Cell Stain other than Toluidine Blue
To: 'hymclab' <hymclab <@t> hyhc.com>, 'Andrea Grantham'
<algranth <@t> u.arizona.edu>, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<DF6AB9298498D31199CF0008C7E6C1200CF95EDA <@t> kalmb02.kaleidahealth.org>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone have a simple Mast Cell Stain other than Toluidine Blue?
Annette Featherstone HT/MLT
-----Original Message-----
From: hymclab [mailto:hymclab <@t> hyhc.com]
Sent: Wednesday, September 29, 2004 15:23
To: 'Andrea Grantham'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Bouins substitute in Trichrome staining
PLEASE POST
-----Original Message-----
From: Andrea Grantham [mailto:algranth <@t> u.arizona.edu]
Sent: Wednesday, September 29, 2004 10:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bouins substitute in Trichrome staining
I attended a workshop at NSH last week on connective tissue staining and
one of the attendees said they were using citrate buffer instead of bouins
as a mordant. I couldn't catch up with her after the workshop to ask about
the protocol. Is anybody out in histoland doing this? Would you mind
sharing your protocol if you are?
Thanks!!
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
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------------------------------
Message: 4
Date: Thu, 30 Sep 2004 04:43:52 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: [Histonet] multi-cassette search
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20040930114352.84514.qmail <@t> web50306.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hello Tracy,
I still use pencil on my cassettes. I have tried all kinds of markers, but
good old #2 lead works best, unless you can get a machine that heat stamps
the # on the cassette.
FYI
NASA researchers have worked for years trying to improve on a pen that
writes in the weightlessness of space, the Russians use pencils. LOL
Paula Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N. Broadway
Moore, OK 73160
405-759-3953
contact <@t> excaliburpathology.com
www.excaliburpathology.com
------------------------------
Message: 5
Date: Thu, 30 Sep 2004 13:16:51 GMT
From: "mprice26 <@t> juno.com" <mprice26 <@t> juno.com>
Subject: [Histonet] RE:Oil & Grease for the Leitz 1512
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20040930.061708.512.646565 <@t> webmail28.nyc.untd.com>
Content-Type: text/plain
Hi Everyone,
I need to know where to order the oil and grease for the Leitz 1512
Microtome.
Thank you.
Marsha Price
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Message: 6
Date: Thu, 30 Sep 2004 08:57:20 -0500
From: Don.Birgerson <@t> leica-microsystems.com
Subject: Re: [Histonet] RE:Oil & Grease for the Leitz 1512
To: "mprice26 <@t> juno.com" <mprice26 <@t> juno.com>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF1561F257.88DF8923-ON86256F1F.004C2280-86256F1F.004CA088 <@t> e-leica.com>
Content-Type: text/plain; charset=us-ascii
Hi Marsha,
Below are the "Leica" numbers for the old Leitz part numbers. Available
from Leica Microsystems or their specimen prep dealers.
Oil No. 601 50ml 14033621783
Leitz #11150000200116 Grease No.411 (white) 8/96 now 14033625075
Leitz #11150000200124 Grease No.410 (brown) 8/96 now 14033624601
Don Birgerson
Leica Microsystems
Technical Assistance Center
Don.Birgerson <@t> Leica-Microsystems.Com
1-800-248-0123 ext 5918
"mprice26 <@t> juno.com" <mprice26
Sent by: To:
histonet <@t> lists.utsouthwestern.edu
histonet-bounces <@t> lists.utsouth cc:
western.edu Subject:
[Histonet] RE:Oil & Grease for the Leitz 1512
09/30/2004 08:16 AM
Hi Everyone,
I need to know where to order the oil and grease for the Leitz 1512
Microtome.
Thank you.
Marsha Price
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Message: 7
Date: Thu, 30 Sep 2004 08:35:52 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] Red chromogens
To: Rena <RFail <@t> Charleston.net>, Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20040930082919.01b01148 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
A talk with DAKO technical services can also help you with their
chromogens. There is also a endog alk phos quenching solution from KPL,
called Universal Blocker, and is used in very beginning of the staining
protocol. It is ready to use and very nice, short, and very convenient. If
you use this, levamisole is not needed (added to chromogen substrate which
with ready to use chromogens, always worried me as a dilution factor) - in
fact we tried the universal block in conjunction with levamisole to see
effects and noticed lessening of red color or signal, not what we
wanted. So you would have to use one or the other. I suggest you try the
KPL blocker -
If you are running your primary antibody at the same concentration for all
these chromogens, you will notice a difference. This is something Chris
van der Loos teaches in his multiple staining workshop but still applicable
to single IHC work - about the efficiency of chromogens compared to
antibody titer. With DAB you would have less conc primary than with new
fuchsin, although Permanent red is approaching DAB for efficiency, some
would call it sensitivity.
At 07:30 PM 9/29/2004, you wrote:
>I have been having some trouble with some Abs stained with New Fuchsin
>and permanent red. Recently I was asked if I were using Levamisole in
>the permanent red and if so how much? I was told too much Levamisole in
>permanent red would result in no staining. I have used 1 drop per ml for
>both New Fuchsin and permanent red, which is the appropriate amount. We
>have run both DABS and either permanent red or New Fuchsin with the
>same AB from the same vial with vastly different results. Any ideas?
>Rena Fail
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 8
Date: Thu, 30 Sep 2004 09:38:27 -0500
From: "Charles Scouten" <cwscouten <@t> myneurolab.com>
Subject: RE: [Histonet] Sucrose cryoprection
To: Pablo S?nchez Quinteiro <psanquin <@t> lugo.usc.es>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AF6F2F09D9DBBB4A9964C6BE5C8EC0FCDD6116 <@t> tpiserver03.Coretech-holdings.com>
Content-Type: text/plain; charset="iso-8859-1"
It does sound like the tissue is not fixed, although 48 hours in
Paraformaldehyde should be sufficient even without perfusion. Is the
solution fully formed? Did any white powder remain in the bottom? It seems
you are not getting good fixative.
Did you prewash with saline or sucrose, or just perfuse? Blood can block
many capillaries and block a through perfusion.
Cordially,
Charles W. Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300
FAX 314 522 0377
cwscouten <@t> myneurolab.com
http://www.myneurolab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pablo
Sánchez Quinteiro
Sent: Thursday, September 30, 2004 4:43 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Sucrose cryoprection
Thank for your help Gayle,
I am sorry my mail was not detailed enough.
Yes, I am doing frozen sections with a freezing microtome. The animals are
perfused with Paraformaldehyde 4% and postfixed for 48 hours in the same
fixative. Then they are transfered to Phosphate buffer.
I have problems when cutting the tissue. The sections (60 microns thick) are
apparently nice, but just after putting them into the buffer they do not
stay flat and they fall to pieces after gentle touching with the paintbrush.
I have seen that after sinking in sucrose the samples show a gelatin-like
appearance. They seem swollen and they are transparent. Could be this
related to a poor fixation?
For immunohistochemistry it is not recommended posfixation times greater tan
24 hours, but maybe postnatal or fetal material need longer postfixation
time.
Thanks for your interest.
Pablo
>We need more details on exactly what you are doing?????? and what the
>problems are? Are you using a cryostat?
>
>At 11:14 AM 9/29/2004, you wrote:
>>Dear listers,
>>
>>I am cutting at the sliding microtome fetal and early postantal mice
>>brains. Could somebody tell me if is it normal that after sucrose
>>cryoprection the pieces of tissue show a gelatin-like appearance? I
>>have lot of problems cutting this tissue.
>>
>>Thanks in advance
>>
>>Pablo
>>
>>
>>
>>
>>_______________________________________________
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)
>
>
>
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------------------------------
Message: 9
Date: Thu, 30 Sep 2004 15:40:20 +0100
From: "Anila Syed" <syedab <@t> totalise.co.uk>
Subject: Re: [Histonet] Sucrose cryoprection
To: <Histonet <@t> lists.utsouthwestern.edu>, Pablo S?nchez Quinteiro
<psanquin <@t> lugo.usc.es>
Message-ID: <002301c4a6fb$695b26c0$14c401a3 <@t> clneuro.ox.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
I used to put my tissue in gradually increasing concs of sucrose for up to 1
week *before* cutting them. But my samples were whole human brainstems, so
your perfused animals may require less time.
I used to try to get 50 micron thick sections of brainstem.
Hope that helps?
Anila
---- Original Message -----
From: "Pablo Sánchez Quinteiro" <psanquin <@t> lugo.usc.es>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, September 30, 2004 10:42 AM
Subject: Re: [Histonet] Sucrose cryoprection
> Thank for your help Gayle,
>
> I am sorry my mail was not detailed enough.
>
> Yes, I am doing frozen sections with a freezing microtome. The animals are
> perfused with Paraformaldehyde 4% and postfixed for 48 hours in the same
> fixative. Then they are transfered to Phosphate buffer.
>
> I have problems when cutting the tissue. The sections (60 microns thick)
> are apparently nice, but just after putting them into the buffer they do
> not stay flat and they fall to pieces after gentle touching with the
> paintbrush.
>
> I have seen that after sinking in sucrose the samples show a gelatin-like
> appearance. They seem
> swollen and they are transparent. Could be this related to a poor
fixation?
>
> For immunohistochemistry it is not recommended posfixation times greater
> tan 24 hours, but maybe postnatal or fetal material need longer
> postfixation time.
>
> Thanks for your interest.
>
> Pablo
>
> >We need more details on exactly what you are doing?????? and what the
> >problems are? Are you using a cryostat?
> >
> >At 11:14 AM 9/29/2004, you wrote:
> >>Dear listers,
> >>
> >>I am cutting at the sliding microtome fetal and early postantal mice
> >>brains. Could somebody tell me if is it normal that after sucrose
> >>cryoprection the pieces of tissue show a gelatin-like appearance? I have
> >>lot of problems cutting this tissue.
> >>
> >>Thanks in advance
> >>
> >>Pablo
> >>
> >>
> >>
> >>
> >>_______________________________________________
> >>Histonet mailing list
> >>Histonet <@t> lists.utsouthwestern.edu
> >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >Gayle Callis
> >MT,HT,HTL(ASCP)
> >Research Histopathology Supervisor
> >Veterinary Molecular Biology
> >Montana State University - Bozeman
> >PO Box 173610
> >Bozeman MT 59717-3610
> >406 994-6367 (lab with voice mail)
> >406 994-4303 (FAX)
> >
> >
> >
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Message: 10
Date: Thu, 30 Sep 2004 08:48:58 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] Mast Cell Stain other than Toluidine Blue
To: "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20040930084605.01b136a8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
No, but I have two methods that are very simple, both are T blue. Churkian
Schenk is my favorite, but have also used a method nicely passed on to me
by Liz Chlipala out of Frieda Carson's book, a pH 4.3 T blue that takes 3
to 5 minutes, rinse with water, air dry the section and mount. This one is
really simple and fast. If you want, I can forward both to you privately.
At 05:37 AM 9/30/2004, you wrote:
>Does anyone have a simple Mast Cell Stain other than Toluidine Blue?
>Annette Featherstone HT/MLT
>-----Original Message-----
>From: hymclab [mailto:hymclab <@t> hyhc.com]
>Sent: Wednesday, September 29, 2004 15:23
>To: 'Andrea Grantham'; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] Bouins substitute in Trichrome staining
>
>
>PLEASE POST
>
>-----Original Message-----
>From: Andrea Grantham [mailto:algranth <@t> u.arizona.edu]
>Sent: Wednesday, September 29, 2004 10:38 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Bouins substitute in Trichrome staining
>
>
>I attended a workshop at NSH last week on connective tissue staining and
>one of the attendees said they were using citrate buffer instead of bouins
>as a mordant. I couldn't catch up with her after the workshop to ask about
>the protocol. Is anybody out in histoland doing this? Would you mind
>sharing your protocol if you are?
>Thanks!!
>Andi Grantham
>.....................................................................
>: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
>: Sr. Research Specialist University of Arizona :
>: (office: AHSC 4212) P.O. Box 245044 :
>: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
>: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
>:...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>_______________________________________________
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>
>CONFIDENTIALITY NOTICE:
>This email transmission and any documents, files,
>or previous e-mail messages attached to it are
>confidential and intended solely for the use of the
>individual or entity to whom they are addressed.
>If you are not the intended recipient, or a person
>responsible for delivering it to the intended recipient,
>you are hereby notified that any further review,
>disclosure, copying, dissemination, distribution, or
>use of any of the information contained in or attached
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>ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777.
>
>
>
>_______________________________________________
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 11
Date: Thu, 30 Sep 2004 12:12:09 -0400
From: "Martha Ward" <mward <@t> wfubmc.edu>
Subject: [Histonet] FXIII antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<61135F0455D33347B5AAE209B903A304076A4F6A <@t> EXCHVS2.medctr.ad.wfubmc.edu>
Content-Type: text/plain; charset="US-ASCII"
I am looking for a vendor for the FXIIIa antibody. I tried DAKO,
Calbiochem and Biogenex and no one carries it. Any help will be
appreciated.
Martha Ward
Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
------------------------------
Message: 12
Date: Thu, 30 Sep 2004 11:29:22 -0500
From: "Jeff Gordon" <JGordon <@t> cellmarque.com>
Subject: RE: [Histonet] FXIII antibody
To: "Martha Ward" <mward <@t> wfubmc.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AE107C473C4B954E80A12057A86FCA834A46CB <@t> cmmail.cellmarque.local>
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Based on the vendors that you have inquired upon that do not offer it, I'm
not sure which companies carry (or carried) Factor 13a, but we do have it at
Cell Marque, if that is an option for you. It is available as both a
concentrate and a ready-to-use.
Jeff Gordon
Cell Marque Corp.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Martha
Ward
Sent: Thursday, September 30, 2004 11:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FXIII antibody
I am looking for a vendor for the FXIIIa antibody. I tried DAKO,
Calbiochem and Biogenex and no one carries it. Any help will be
appreciated.
Martha Ward
Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
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Message: 13
Date: Thu, 30 Sep 2004 09:31:57 -0700
From: May Wei <m.wei <@t> biogenex.com>
Subject: RE: [Histonet] FXIII antibody
To: 'Martha Ward' <mward <@t> wfubmc.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<C6D69284EF18D511B53800E018C10A9F03F83E21 <@t> isleton.biogenex.com>
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Dear Martha,
BioGenex (1-800-421-4149) has released this Factor XIIIa antibody recently.
It is monoclonal with clone [E980.1]. The RTU catalog number is AM337-5M.
The concentrated format catalog number is MU337-UC.
May Wei, M. Med., MBA
International Account Manager
BioGenex Laboratories
4600 Norris Canyon Road
San Ramon, CA 94583
Tel: (925) 543-1350
Fax: (925) 866-2525
Email: m.wei <@t> biogenex.com
-----Original Message-----
From: Martha Ward [mailto:mward <@t> wfubmc.edu]
Sent: Thursday, September 30, 2004 9:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FXIII antibody
I am looking for a vendor for the FXIIIa antibody. I tried DAKO,
Calbiochem and Biogenex and no one carries it. Any help will be
appreciated.
Martha Ward
Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
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------------------------------
Message: 14
Date: Thu, 30 Sep 2004 10:33:44 -0600
From: "Jason and Heather" <themagoos <@t> rushmore.com>
Subject: [Histonet] FXIII antibody
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001e01c4a70b$427c7cb0$b3fc9fd1 <@t> magoo>
Content-Type: text/plain; charset="iso-8859-1"
We use a mouse antibody from Cell Marque. We have had good luck with it.
Jason McGoughHT(ASCP)
Histology
Clinical Lab of the Black Hills
------------------------------
Message: 15
Date: Thu, 30 Sep 2004 11:32:19 -0500
From: "Poteete, Jacquie A." <japoteete <@t> saintfrancis.com>
Subject: RE: [Histonet] FXIII antibody
To: 'Martha Ward' <mward <@t> wfubmc.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<D423BBB85E91D411A38D42003000996506973EC2 <@t> mailnt1.saintfrancis.com>
Content-Type: text/plain
We use CellMarque CMC780 Factor XIIIa with good results. Their number is
1-800-665-7284.
Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Laboratory
Saint Francis Hospital, Tulsa, OK
japoteete <@t> saintfrancis.com
> -----Original Message-----
> From: Martha Ward [SMTP:mward <@t> wfubmc.edu]
> Sent: Thursday, September 30, 2004 11:12 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] FXIII antibody
>
> I am looking for a vendor for the FXIIIa antibody. I tried DAKO,
> Calbiochem and Biogenex and no one carries it. Any help will be
> appreciated.
>
> Martha Ward
> Molecular Diagnostics Lab
> Wake Forest University Baptist Medical Center
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
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