[Histonet] Mast Cell Stain other than Toluidine Blue

Gayle Callis gcallis <@t> montana.edu
Thu Sep 30 09:48:58 CDT 2004

No, but I have two methods that are very simple, both are T blue.  Churkian 
Schenk is my favorite, but have also used a method nicely passed on to me 
by Liz Chlipala out of Frieda Carson's book, a pH 4.3 T blue that takes 3 
to 5 minutes, rinse with water, air dry the section and mount.  This one is 
really simple and fast.   If you want, I can forward both to you privately.

At 05:37 AM 9/30/2004, you wrote:
>Does anyone have a simple Mast Cell Stain other than Toluidine Blue?
>Annette Featherstone HT/MLT
>-----Original Message-----
>From: hymclab [mailto:hymclab <@t> hyhc.com]
>Sent: Wednesday, September 29, 2004 15:23
>To: 'Andrea Grantham'; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] Bouins substitute in Trichrome staining
>-----Original Message-----
>From: Andrea Grantham [mailto:algranth <@t> u.arizona.edu]
>Sent: Wednesday, September 29, 2004 10:38 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Bouins substitute in Trichrome staining
>I attended a workshop at NSH last week on connective tissue staining and
>one of the attendees said they were using citrate buffer instead of bouins
>as a mordant. I couldn't catch up with her after the workshop to ask about
>the protocol. Is anybody out in histoland doing this? Would you mind
>sharing your protocol if you are?
>Andi Grantham
>: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
>: Sr. Research Specialist       University of Arizona               :
>: (office:  AHSC 4212)          P.O. Box 245044                     :
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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