[Histonet] RE: RE: Recycled Alcohol and Xylene

[Aldo Anile]

Just a passing comment. When recycling xylene, in most cases the purity is in fact greater than the xylene you commercially buy, so using recycled xylene should not impact at all on processing or staining.

When recycling alcohol, because of the chemistry involved in the distillation and the affinities of alcohol, at best only approx 96% alcohol is ever achieved through recyling. If using recycled alcohol in processing I suggest several changes of absolute alcohol at the end of your dehydration step. Recycled alcohol is best only used up to the 95% alcohol dehydrating steps in processing.

Aldo Anile 
Applications Consultant

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