[Histonet] re vimentin on mouse tissue

[Kim Merriam]

I agree with Jason.  At my former job, we used to use the DAKO V9 clone to stain human cells that had been implanted into mouse tissue.  We used it because it did not cross-react with the mouse tissue (we wanted to only see the human cells).
 
Just my 2cents.
 
Kim Merriam
Novartis
Cambridge, MA

"PALMER Jason (SVHM)" <Jason.PALMER <@t> svhm.org.au> wrote:


We use Dako vimentin V9 as a human specific marker in human / mouse hybrid tissue. We have never ever seen it stain mouse tissue of any sort, and that's the way we like it! So, I'm not sure that you'll get it to stain (though I think I remember somebody on histonet in the past claiming that it does work with mouse....).

Cheers,

Jason

Jason Palmer
Bernard O'Brien Institute of Microsurgery 
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: palmerj <@t> svhm.org.au



Message: 13
Date: Mon, 27 Sep 2004 15:55:06 -0700 (PDT)
From: Yolanda Maldonado 
Subject: [Histonet] Vimentin on mouse tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20040927225506.66447.qmail <@t> web53507.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hi everyone:
Does anyone out there has any experience with IHC stain for Vimentin on mouse tissue?
I have tried the V9 and 3B4 clone from different companies as well as the ARK kit and other polymers...Nothing seems to work for me!!
I have tried EDTA. CB, Reveal, LAB and enzymes as AR and I have also run human and other species along with my tissue to make sure my staining is working. Everything stains but the mice.
Any suggestions?


Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Kim Merriam
Novartis
Cambridge, MA
		
---------------------------------
Do you Yahoo!?
New and Improved Yahoo! Mail - 100MB free storage!