[Histonet] (no subject)
gcallis <@t> montana.edu
Tue Sep 28 11:18:29 CDT 2004
In reply to using fluorophore labelled primary antibodies, you can still
use these for immunohistochemistry if you just come back with a secondary
antibody that is anti FITC, antiPE, etc. DAKO has a rabbit antiFITC that
is excellent, there are others on the markey. If you want to get away from
a biotin systems, you can always use a polymer immunostaining system to
detect the rabbit antiFITC.
It should work very well. Sorry I can't help you with antibody source.
Collagen IV subtypes: alpha 3-alpha 6,
Does anyone know where I can get antibodies to subtypes of all collagen IV
chains (alpha 1alpha 6)?
Are they only available for immunofluorescence detection?
SantaCruz has some antibodies for collagen IV alpha 1, alpha 2 and alpha 3
isoforms. I also know that ID Labs in Canada has a clone specific to alpha 1
and alpha 2.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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