[Histonet] Processing lung tissue after pulmonary function tests

Randolph-Habecker, Julie jhabecke <@t> seattlecca.org
Mon Sep 27 11:11:52 CDT 2004


I am working with an investigator who is studying lung function after lung
transplantation. We would like to look at FFPE lung tissue after the subject
has had lung function tests which involve fluorescent plastic beads of
varying size - the largest being 15 microns. I am concerned that the normal
processing could melt the beads and cause artifact. Someone in another lab
suggested that we use Histoclear II rather than Xylene.

Does anyone have experience in processing tissue like this? Please give me
some information on how to handle this tissue.



Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345
jhabecke <@t> fhcrc.org <mailto:jhabecke <@t> fhcrc.org>

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