[Histonet] IHC - weak signals

[Greg Dobbin]

Hi Jason,
Post more details about your procedure. The chromagen step is 
only one of many steps that can contribute to signal strength, and at 
that, not the most likely place to start. Tell the group what you are 
staining for, with frozens or paraffin sections, how long and at what 
temp the primary is applied, with what detection system, if any 
retreival methods are being employed, etc. We need the whole 
story before we can offer any help.
Cheers!
Greg

From:           	"jason m" <kosmicdog <@t> hotmail.com>
To:             	histonet <@t> pathology.swmed.edu
BCC to:         	Date sent:      	Fri, 24 Sep 2004 07:19:25 -0700
Copies to:      	Subject:        	[Histonet] IHC - weak signals

> Does anyone have advice for amplifying weak signals from IHC? Is there a 
> detection system better than DAB which can make the signal more noticable?
> 
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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Happiness is a journey, not a destination.