[Histonet] Water bath antigen retrieval

Paula Pierce contact <@t> excaliburpathology.com
Thu Sep 23 13:04:32 CDT 2004

I use a Black and Decker rice steamer for antigen retrieval. About $30.
Fill the bottom with distilled water.
Place the plastic basket in the steamer.
Place a container with your slides and citrate buffer in the basket so the lid will fit.
Turn the steamer on for 1 hour, then let cool for 25 minutes.
Rinse in distilled water slowly to finish cooling.
Proceed with your IHC stain.

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