[Histonet] re: cell culture

[Vonk, M.J. (KNO)]

Hello Galina and histonetters,

I also tried to do histology on transwell inserts by Costar.
I also followed the protocol but it did not work for me.
The problem is that the paraffin does not stick to the membrane. What I got
when I removed the membrane plug was that the membrane got separated from
the paraffin. My cells are now partially on the membrane and partially in
the paraffin plug.

Has anyone got some ideas about how to embed such a transwell insert without
destroying my cells?

Marcel Vonk


*******************************************
M.J.Vonk
Leiden University Medical Center
Dept of Otorhinolaryngology
Room J2-77
Albinusdreef 2
PO-Box 9600
2300 RC
Leiden
The Netherlands
tel: ..3171-5261179
M.J.Vonk <@t> lumc.nl


Message: 5
Date: Mon, 20 Sep 2004 13:07:01 -0700 (PDT)
From: Galina Deyneko <galinadeyneko <@t> yahoo.com>
Subject: [Histonet] cell culture
To: Stephen.Eyres <@t> sanofi-synthelabo.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20040920200701.52880.qmail <@t> web14525.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Steve,
web-site www.corning.com/lifesciences and find Preparation of Costar
Transwell Inserts for Histology. Follow this protocol . I did it  and
received good results. call me if you have questions.
Galina Deyneko
Novartis
617-871-7613